Paulien Cornelissen - Steijger

Technician
Team Member

Short CV

2017-presentResearch TechnicianVU University Medical Center Amsterdam, NL
2007-2016Research Technician, cancer researchNKI-AVL Amsterdam, NL
1993-2007Research Technician, DNA repair researchLUMC, Leiden, NL
1980-1985Research Technician, clinical immunologySSDZ, Delft, NL
1993HLO BiochemistryHvA Amsterdam, NL
1980HBO-B Biochemical TechnicianVLI, Delft, NL

Experience

In the laboratory of the hospitals in Delft I did T-and B celltyping and and HLA typing, immunofluorescence and radio immuno assays.
During research in the LUMC we worked on enhanced reactivation of DNA repair-deficient cells. We also studied signaaltransduction pathways in DNA repair deficient fibroblasts. Therefor we used several techniques as: tissue culture, transfections, transductions, Western- and Northernblotting
The last 10 years I worked first in the lab of Prof M.van Lohuizen on the function of polycombproteins in breast, prostate and intestinal cancer. Later I participated in the lab of prof. D.Peeper and there we did research on skin cancer. Besides the techniques mentioned before we did also mini/maxipreps, immunohistochemistry, QPCR.

Contact info

Address:

VU University
De Boelelaan 1085, W&N Bldg, Room G350
1081 HV Amsterdam
The Netherlands

Telephone:

+31(0)20 5981820

E-mail:p.cornelissen@vumc.nl