Lisa Gasparotto

Technician
Team Member

Short CV

• 2016 – present Research Technician Stem Cell lab, VU/UMC University Amsterdam
• 2008 – 2015 Biologist, Private human medical analysis laboratory, Italy
• 2005 – 2008 Biologist, Veterinary hospital laboratory, Italy
• 2003 – 2005 Scientific researcher, Padua University, Department of Biomedical Science
• 2003 Master Degree in Biological Sciences, Padua University, Italy

Research / responsibilities

My work as lab technician of the Stem Cell lab is mainly focus on cell culture. I am part of the team that is taking care of the process of making IPSC (induced pluripotent stem cells) starting from patient’s derived fibroblasts or blood. I also differentiate the IPSC towards neurons, astrocytes, oligodendrocytes and microglia cells. Currently I am working on setting protocols for culturing the different types of cells that we differentiate form IPSC together into 2D or 3D culture systems. I am also involved in the generation of brain organoids. 

Highlighted publications

• Astrocyte Subtype Vulnerability in Stem Cell Models of Vanishing White Matter. Leferink PS, Dooves S, Hillen AEJ, Watanabe K, Jacobs G, Gasparotto L, Cornelissen-Steijger P, van der Knaap MS, Heine VM. Ann Neurol. 2019 Nov;86(5):780-792. doi: 10.1002/ana.25585. Epub 2019 Sep 10.PMID: 31433864

• Neuron-Glia Interactions Increase Neuronal Phenotypes in Tuberous Sclerosis Complex Patient iPSC-Derived Models. Nadadhur AG, Alsaqati M, Gasparotto L, Cornelissen-Steijger P, van Hugte E, Dooves S, Harwood AJ, Heine VM. Stem Cell Reports. 2019 Jan 8;12(1):42-56

• Patterning factors during neural progenitor induction determine regional identity and differentiation potential in vitro. Nadadhur AG, Leferink PS, Holmes D, Hinz L, Cornelissen-Steijger P, Gasparotto L, Heine VM. Stem Cell Res. 2018 Oct;32:25-34

• Cytologic detection of Call-Exner bodies in Sertoli cell tumors from 2 dogs. Masserdotti C, De Lorenzi D, Gasparotto L. Vet Clin Pathol. 2008 Mar;37(1):112-4

• Human amniotic fluid-derived stem cells are rejected after transplantation in the myocardium of normal, ischemic, immuno-suppressed or immuno-deficient rat. Chiavegato A, Bollini S, Pozzobon M, Callegari A, Gasparotto L, Taiani J, Piccoli M, Lenzini E, Gerosa G, Vendramin I, Cozzi E, Angelini A, Iop L, Zanon GF, Atala A, De Coppi P, Sartore S. J Mol Cell Cardiol. 2007 Apr;42(4):746-59

• Amniotic fluid and bone marrow derived mesenchymal stem cells can be converted to smooth muscle cells in the cryo-injured rat bladder and prevent compensatory hypertrophy of surviving smooth muscle cells. De Coppi P, Callegari A, Chiavegato A, Gasparotto L, Piccoli M, Taiani J, Pozzobon M, Boldrin L, Okabe M, Cozzi E, Atala A, Gamba P, Sartore S. J Urol. 2007 Jan;177(1):369-76

• Differential availability/processing of decorin precursor in arterial and venous smooth muscle cells. Franch R, Chiavegato A, Maraschin M, Candeo S, Ausoni S, Villa A, Gerosa G, Gasparotto L, Parnigotto P, Sartore S. J Anat. 2006 Sep;209(3):271-87

• Amniotic mesenchymal cells autotransplanted in a porcine model of cardiac ischemia do not differentiate to cardiogenic phenotypes. Sartore S, Lenzi M, Angelini A, Chiavegato A, Gasparotto L, De Coppi P, Bianco R, Gerosa G. Eur J Cardiothorac Surg. 2005 Nov;28(5):677-84

• Role of platelet activation in catheter-induced vascular wall injury. Zolpi E, Filipetto C, Bertipaglia B, Taiani J, Gasparotto L, Chiavegato A, Gamba P, Sartore S. J Endovasc Ther. 2004 Apr;11(2):196-210.

Contact info

Address:

Vrije Universiteit Amsterdam
Center for Neurogenomics and Cognitive Research (CNCR)
Department of Complex Trait Genetics
W&N building, Room G-349
De Boelelaan 1085
1081 HV Amsterdam
The Netherlands

Telephone:

+31 20 598 85 03

E-mail:l.gasparotto@amsterdamumc.nl