Lisa Gasparotto

Technician

Short CV

2016-Research Technician Stem Cell labAmsterdam UMC / VU University Amsterdam
2008-2015BiologistPrivate human medical analysis laboratory, Italy
2005-2008BiologistVeterinary hospital laboratory, Italy
2003Master Degree in Biological SciencesPadua University, Italy

Responsibilities

My work as lab technician of the Stem Cell lab is mainly focus on cell culture. I am involved in the process of making IPSC, starting mainly from patient’s derived fibroblasts, of expansion, maintenance and on characterization of these cells. I am aIso taking care of the differentiation of the IPSC towards neurons, astrocytes, oligodendrocytes and microglia cells. Currently I am working on setting protocols for making brain organoids. 

Publications

Neuron-glia interactions increase neuronal phenotupes in Tuberous Sclerosis Complex patient iPSC-derived models. Nadadhur AG, Alsaqati M, Gasparotto L, Cornelissen-Steijger P, van Hugte E, Dooves S, Harwood AJ, Heine VM. Stem Cell Reports, 2019

Patterning factors during neural progenitor induction determine regional identity and differentiation potential in vitro. Nadadhur AG, Leferink PS, Holmes D, Hinz L, Cornelissen-Steijger P, Gasparotto L, Heine VM. Stem Cell Res, 2018

Cytologic detection of Call-Exner bodies in Sertoli cells tumors from 2 dogs. Masserdotti C, De Lorenzi D, Gasparotto L. Vet Clin Pathol, 2008.

Human amniotic fluid-derived stem cells are rejected after transplantation in the myocardium of normal, ischemic, immuno-suppressed or immune-deficient rat. Chiavegato A, Bollini S, Pozzobon M, Callegari A, Gasparotto L, Taiani J, Piccoli M, Lenzini E, Gerosa G, Vendramin I, Cozzi E, Angelini A, Iop L, Zanon GF, Atala A, De Coppi P, Sartore S. Journal of molecular and cellular cardiology, 2007.

Amniotic fluid and bone marrow derived mesenchymal stem cells can be converted to smooth muscle cells in the cryo-injured rat bladder and prevent compensatory hypertrophy of surviving smooth muscle cells. De Coppi P, Callegari A, Chiavegato A, Gasparotto L, Piccoli M, Taiani J, Pozzobon M, Boldrin L, Okabe M, Cozzi E, Atala A, Gamba P, Sartore S. The Journal of Urology, 2007.

Differential availability/processing of decorin precursor in arterial and venous smooth muscle cells. Franch R, Chiavegato A, Maraschin M, Candeo S, Ausoni S, Villa A, Gerosa G, Gasparotto L, Parnigotto P, Sartore S. J Anat, 2006

Amniotic mesenchymal cells autotransplanted in a porcine model of cardiac ischemia do not differentiate to cardiogenic phenotypes. Sartore S, Lenzi M, Angelini A, Gasparotto L, De Coppi P, Bianco R, Gerosa G. Eur J Cardiothorac Surgery, 2005.

Role of platelet activation in catheter-induced vascular wall injury. Zolpi E, Filippetto C, Bertipaglia B, Taiani J, Gasparotto L, Chiavegato A, Gamba P, Sartore S. J Endovasc Therapy, 2004

Contact info

Address:

VU University
De Boelelaan 1085, W&N Bldg, Rm G349
1081 HV Amsterdam
The Netherlands

Telephone:

+31(0)20 598 85 03

E-mail:l.gasparotto@amsterdamumc.nl